Fig. 6

Elevated cPLA₂α protein expression in the motor neurons is highly correlated with TNFα. A Double immunofluorescence staining of cPLA₂α (green) and TNFα (red) proteins in the lumbar spinal cord sections of WT and 6 weeks old mutant SOD1^G93A mice. Scale bars = 100 μm. B The Pearson coefficient correlation (r) between cPLA₂α and TNFα in the spinal motor neurons of mutant SOD1^G93A mice was analyzed. Florescence intensity is expressed in arbitrary units of immunostaining as presented in the representative results in A. Four fields in each of the 8 different mice analyzed. C Elevated cPLA₂α and TNFα in primary motor neurons expressing mutant SOD1^G93A. Double immunofluorescence staining of cPLA₂α (green) and TNFα (red) in primary motor neurons expressing human SOD1^WT, mutant SOD1^G93A and control cells described in Fig. 3. Two upper panels, scale bars = 50 μm and two lower panels, scale bar s = 20 μm. 3 different independent experiments were analyzed and showed similar results. The means ± SEM fluorescence intensity for cPLA₂α and TNFα is presented in the bar graphs as arbitrary units. Five fields in each of the 3 different treatments of motor neurons in each experiment was analyzed. Significance compared to control ***p < 0.001, n.s. non-significant