Fig. 2

OPTN alleviates Aβo-induced dysfunction of mitochondrial autophagy. A, B BV2 cells were treated with Aβo in the absence or presence of transfection with plvx-IRES-OPTN. A mKeima fluorescence was evoked using two excitation filters (438 ± 12 nm and 550 ± 15 nm) and a 610LP emission filter. B Expression levels of HSP60 and OPTN were detected by western blotting. β-actin served as an internal control. The data represent the means ± S.E. of independent experiments. BV2 cells treated with vehicle group or Aβo and overexpressing OPTN were compared with group Aβo alone *P < 0.05, **P < 0.01, ***P < 0.001