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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Downregulating expression of OPTN elevates neuroinflammation via AIM2 inflammasome- and RIPK1-activating mechanisms in APP/PS1 transgenic mice

Fig. 4

Aβo activates the AIM2 inflammasome. A–D BV2 cells were treated with Aβo for 12 h. A Expression levels of AIM2, ASC, pro-caspase-1 and caspase-1 were detected by western blotting. β-actin served as an internal control. B ImageJ software was used for semiquantitative analysis of western blots. C qRT-PCR was used to detect the mRNA expression of AIM2 and ASC with GAPDH as an internal control. D Secretion of IL-1β was evaluated by ELISA. E–H Primary microglia were treated with Aβo for 12 h. E Protein levels of AIM2, ASC, pro-caspase-1, and caspase-1 were detected by western blotting with β-actin as the internal control. F ImageJ software was used to semiquantitatively analyze the fold change in AIM2, ASC, pro-caspase-1 and caspase-1 relative to β-actin. G mRNA expression of AIM2 and ASC was detected by qRT-PCR with GAPDH as an internal control. H Secretion of IL-1β was detected by ELISA. The data present means ± S.M. of independent experiment. Aβo treatment were compared with vehicle treatment *P < 0.05, **P < 0.01, ***P < 0.001

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