Fig. 3

Chronic HFD exposure increased circulating concentrations of inflammatory factors. Plasma levels of cytokines and chemokines analyzed at the end of the experiment in unstressed (Controls) or stressed (UCMS) SD and HFD mice. A Heatmap generated with R gplots package showing relative plasma levels (as compared to the control group) of detected inflammatory factors displaying notable differences between groups. Controls are not presented since their value was equal to 0 (white color). The table associated to the heatmap shows results of the statistical analysis reporting the effects of Diet, Stress and their interactions. ^*P < 0.05 ^**P < 0.01, ^***P < 0.001; ns = not significant. B Integrated plasma inflammatory z-score calculated from data displayed in the heatmap and graphed as means ± SEM. n = 8–15 mice/group. ^***P < 0.001 for Diet effect