Fig. 4

HFD and UCMS differentially modulated HC gene expression of inflammatory markers and related neurobiological processes. A Heatmap generated with R gplots package displaying differential expression levels of hippocampus (HC) genes, as analyzed with the TLDA method in unstressed (Controls) or stressed (UCMS) SD and HFD mice. Results are plotted as fold changes relative to controls, which are not presented since their value was equal to 0 (white color). Genes showing poor or late amplification were not included in the analysis. The table associated to the heatmap displays results of the statistical analysis for genes related to: inflammation, KYN pathway, BH4 pathway, 5-HT system, glutamate system and oxidative status. ^*P < 0.05 ^**P < 0.01; ns = not significant. B Inflammatory, C kynurenine, D BH4, E 5-HT, F glutamate and G oxidative z-scores calculated from expression of related genes displayed in the heatmap. All results are graphed as means ± SEM. n = 8–10 mice/group. ^$P ≤ 0.05, ^$$P < 0.01 for differences vs. unstressed-SD mice; ⁺P ≤ 0.05 for differences vs. unstressed-HFD mice