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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Tonicity-responsive enhancer-binding protein promotes diabetic neuroinflammation and cognitive impairment via upregulation of lipocalin-2

Fig. 3

TonEBP haploinsufficiency attenuates systemic inflammation and memory deficits in HFD/STZ-induced diabetic mice. a, b Expressions of mRNAs encoding TNF-α (F = 10.11, p = 0.005), IL-1β (F = 4.594, p = 0.047), Arg1 (F = 0.7228, p = 0.406), and mrc2 (F = 7.333, p = 0.014) in adipose tissues were measured by quantitative RT-PCR (n = 4–6). c, d Circulating TonEBP c (F = 4.3, p = 0.053) and LCN2 d (F = 18.12, p < 0.001) levels were measured using ELISAs (n = 5–6). e Western blots and quantified adipose tissue expression of TonEBP (F = 35.91, p < 0.001) and LCN2 (F = 10.07, p = 0.009) (n = 3–4). f Representative H&E and immunostaining images for TonEBP and LCN2 (n = 3–4). g Quantification of TonEBP (F = 4.522, p = 0.046) or LCN2 (F = 57.99, p < 0.001) -positive cell counts in the images (n = 3–4). Data are shown as mean ± SEM. The indicated p values represent a two-way ANOVA followed by Tukey’s post-hoc test. *p < 0.05 versus WT ND. p < 0.05 versus WT HFD/STZ

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