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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Tonicity-responsive enhancer-binding protein promotes diabetic neuroinflammation and cognitive impairment via upregulation of lipocalin-2

Fig. 5

TonEBP haploinsufficiency attenuates hippocampal LCN2 and HO-1 expression in HFD/STZ-induced diabetic mice. a Western blots and quantified hippocampal expression of TonEBP (F = 4.977, p = 0.04) and LCN2 (F = 6.447, p = 0.019). Band intensities were normalized to β-actin (n = 4–7). b Representative immunofluorescence staining for LCN2 in the hippocampal CA1 region sections. Quantification of LCN2-immunostained density in the images (n = 3–4, F = 22.83, p < 0.001). c Western blots and quantified hippocampal expression of HO-1 (n = 4–5, F = 23.54, p < 0.001). Band intensities were normalized to β-actin. d Representative double immunofluorescence staining for TonEBP and HO-1 in hippocampal CA1 region sections. Quantification of co-localized TonEBP and HO-1-immunostained density in the images (n = 3–4, F = 2.525, p = 0.125). Data are shown as mean ± SEM. The indicated p values represent a two-way ANOVA followed by Tukey’s post-hoc test. *p < 0.05 versus WT ND. p < 0.05 versus WT HFD/STZ

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