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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Chemokine CCL2 prevents opioid-induced inhibition of nociceptive synaptic transmission in spinal cord dorsal horn

Fig. 2

Effects of SB366791, CCL2, and minocycline on DAMGO induced depression and delayed potentiation of the eEPSC amplitude. A, E Application of DAMGO (1 µM, 3 min, n = 15) decreased the eEPSC amplitude in all of the recorded dorsal horn neurons. This depression was followed by an increase of eEPSC amplitude during the wash-out in 9 out of the 15 neurons. B, E Pre-treatment with TRPV1 antagonist SB366791 (10 µM, 4 min, n = 9) prevented DAMGO-induced increase of eEPSC amplitude during the washout. C, F Pre-incubation of spinal cord slices with CCL2 (10 nM, 2 h, n = 11) prevented both the DAMGO-induced decrease and the delayed increase of the eEPSC amplitude. D, F Inhibition of microglial activation with minocycline (100 µM, n = 8) prevented the blocking effect of CCL2 on DAMGO-induced decrease of the eEPSC amplitude. Statistical differences in each experimental group were identified using RM ANOVA followed by the Bonferroni test; *P < 0.05, **P < 0.01, ***P < 0.001 versus Ctrl/SB366791 part of the recording. Differences between treatments were analysed using one-way ANOVA followed by the Student–Newman–Keuls test; ˄P < 0.05, ˄˄P < 0.01, ˄˄˄P < 0.001 versus CTRL (E) or CCL2 group (F); +P < 0.05 versus minocycline group

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