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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Dopaminergic stimulation leads B-cell infiltration into the central nervous system upon autoimmunity

Fig. 3

Drd3 deficiency in B cell abrogates disease manifestation in an EAE model that depends on the APC-function of B cells. BM chimeric mice harbouring Drd3-deficient (grey symbols) or Drd3-sufficient (black symbols) B cells were generated as described in Fig. 2A. Next, EAE was induced in chimeric mice by immunization with huMOG in CFA followed by pertussis toxin injection. A Disease severity was evaluated throughout the time-course of the disease development. Values represent the mean ± SEM; n = 4 mice per group. B–D At the peak of disease severity (day 15 post-induction), mononuclear cells were isolated from the CNS (B and C) and the spleen (D) followed by ex vivo stimulation with PMA/ionomycin in the presence of brefeldin A, and intracellular cytokine staining analysis in CD4+ Tcells was carried out by flow cytometry. B Representative dot-plots in the CD4+ gate are shown. Numbers indicate the percentage of cells in the corresponding quadrant. C, D Quantification of the frequency of CD4+ T cells producing IFNγ, IL-17, GM-CSF or expressing FoxP3. n = 3–4 mice per group. Each symbol represents data obtained from an individual mouse. The mean ± SEM are depicted. Data representative from one out of two independent experiments are shown. *, p < 0.05; **, p < 0.01; ****, p < 0.0001 by Mann–Whitney U test (A) or two-way ANOVA followed by Sidak’s post hoc test (C, D)

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