Fig. 3

The impacts of sTREM2 fragments on amyloid deposition in 5xFAD mice. A and D The 7-month-old 5xFAD mice were injected with the Fc-tagged sTREM2 fragment 41–81 or Fc alone into the right and left hippocampi, respectively. A Coronal sections were stained with DAPI (blue) for nuclei, MOAB-2 (green) for Aβ, and Iba1 (red) for microglia. D Coronal sections were stained with Thio-S (blue) for dense-core plaque, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification× 20; scale bar, 100 μm. B Quantitation of the Iba1-positive area in A (n = 8 mice, 24 fields of each group for analysis, paired Student’s t test). C Quantitation of the amyloid plaque deposition in A (n = 8 mice, 24 fields of each group for analysis, paired Student’s t test). E Quantitation of the Thio-S-positive area in D (n = 5 mice, 15 fields of each group for analysis, paired Student’s t test). F The 7-month-old 5xFAD mice were injected with the Fc-tagged sTREM2 fragment 51–81 or Fc alone into the right and left hippocampi, respectively. Coronal sections were stained with DAPI (blue) for nuclei, MOAB-2 (green) for Aβ, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification× 20; scale bar, 100 μm. G Quantitation of the Iba1-positive area in F (n = 8 mice, 24 fields of each group for analysis, paired Student’s t test). H Quantitation of the amyloid plaque deposition in F (n = 8 mice, 24 fields of each group for analysis, paired Student’s t test)