Fig. 5

Comparing the impacts of sTREM2 fragment 41–81 and the full-length protein on amyloid plaque deposition in 5xFAD mice. A and E The 8-month-old 5xFAD mice were injected with the Fc-tagged sTREM2 1–157 or fragment 41–81 into the right and left hippocampi, respectively. A, coronal sections were stained with DAPI (blue) for nuclei, MOAB-2 (green) for Aβ, and Iba1 (red) for microglia. E, coronal sections were stained with Thio-S (blue) for dense-core plaque, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification × 20; scale bar, 100 μm. B Quantitation of the Iba1-positive area in A (n = 5 mice, 15 fields of each group for analysis, paired Student’s t test). C Quantitation of amyloid plaque deposition in A (n = 5 mice, 15 fields of each group for analysis, paired Student’s t test). D Quantitation of the number of plaques with different sizes in A (n = 5 mice, 15 fields of each group for analysis, paired Student’s t test). F Quantitation of the Thio-S-positive area in E (n = 5 mice, 15 fields of each group for analysis, paired Student’s t test). G Coronal sections from the 5xFAD mice injected with the Fc-tagged sTREM2 1–157 and sTREM2 fragment 41–81 were stained with MOAB-2 (green) for Aβ, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification × 60; scale bar, 50 μm. H. Quantitation of the number of plaque-associated microglia in the presence of sTREM2 1–157 or 41–81 (n = 5 mice, 54 plaques of 41–81 and 47 plaques of 1–157, paired Student’s t test). I. Coronal sections from the sTREM2-injected 5xFAD mice were stained with DAPI (blue) for nuclei, MOAB-2 (green) for Aβ and Lamp1 (white) for dystrophic neurites. Representative z-stack images of the hippocampus regions are shown. Original magnification × 20; scale bar, 100 μm. J Quantitation of the total area of Lamp1-positive dystrophic neurites in I (n = 5 mice, 15 fields of each group for analysis, paired Student’s t test)