Fig8

Immunofluorescence staining of hippocampal sections in each group rats (IL-1β) (n = 3, per group). The microglia transform from ameboid form (α7nAchR(−/−) + taVNS group and Hi MLA + taVNS group) to ramified state (Hi saline + taVNS). Compared with α7nAchR(−/−) + taVNS group and Hi MLA + taVNS group, the co-expression between pro-inflammatory cytokine IL-1β (green) and microglia (red) decreased in Hi saline + taVNS group. IL-1β (green), Iba-1 (red), DAPI (blue), Merge (yellow); 40 times objective lens, scale bar: 100 μm and 20 μm magnification