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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Correction to: Inhibiting aberrant p53-PUMA feedback loop activation attenuates ischaemia reperfusion-induced neuroapoptosis and neuroinflammation in rats by downregulating caspase 3 and the NF-κB cytokine pathway

Fig. 3

Double immunofluorescence staining of p53 and PUMA with spinal major cellular markers after IR. a Representative immunofluorescence analysis of the colocalization of neurons (NeuN; green), microglia (Iba1; green) and astrocytes (GFAP; green) with p53 (red) in the anterior horns of grey matter at 48 h after IR. The arrows indicate co-localization with yellow labelling. Scale bars = 50 μm. b Quantification of thep53-positive neurons, astrocytes and microglia was performed and presented as the average of three independent images. Data are expressed as the mean ± SEM. *P < 0.05, versus the sham group. c Representative immunofluorescence analysis of the colocalization of neurons (NeuN; green), microglia (Iba1; green) and astrocytes (GFAP; green) with PUMA (red) in the grey matter of the anterior horn at 48 h after IR with or without si-PUMA treatment. Scale bars = 50 μm. d Quantification of PUMA-positive neurons, astrocytes and microglia was performed and presented as the average of three independent images. Data are expressed as the mean ± SEM. *P < 0.05, versus the sham group. *P < 0.05, versus the IR group

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