Fig. 3

Sevo activates EZH2 expression via FGF2 to repress neurological deficits in TBI rats. A Western blot analysis of EZH2 expression in the cortical tissue of sham-operated, TBI, or Sevo-treated TBI rats. Sevo-treated TBI rats were treated with sh NC, sh FGF2, oe NC, or oe FGF2. B Western blot analysis of EZH2 expression in the cortical tissue of TBI rats. Sevo-treated TBI rats were treated with sh NC, sh EZH2, or sh EZH2 + oe FGF2. C Immunofluorescence analysis of EZH2 expression in the cortical tissue of TBI rats. D Western blot analysis of EZH2 expression in the cortical tissue of TBI rats. E Neurological function assessment by mNSS, brain water content evaluation and motor function score in TBI rats. F BDNF and NeuN expression measured by RT-qPCR and Western blot analysis in the cortical tissue of TBI rats. G Nissl staining of the neuronal damage in the cortical tissue of TBI rats. H TUNEL-positive cells in the cortical tissue of TBI rats. I Protein expression of autophagy-related genes (LC3-I, LC3-II, Beclin-1, and P62) in the cortical tissue of TBI rats detected by Western blot analysis. J Flow cytometric analysis of hippocampal neuronal apoptosis in the cortical tissue of TBI rats. *p < 0.05 vs. sham-operated rats or Sevo-treated TBI rats treated with oe NC or oe NC + sh NC; ^#p < 0.05 vs. TBI rats or Sevo-treated TBI rats treated with sh NC or oe NC + sh EZH2. n = 12 for rats upon each treatment