Fig. 4

SB203580 activates autophagy through TFEB. A, B Lysates from the SNpc of mice were immunoblotted using the indicated antibodies. The protein levels of TFEB, LAMP1 and SQSTM1 (P62) were statistically analyzed in B. Mean ± SEM, n = 6, *p < 0.05. C, D Cell lysates from BV2 cells were immunoblotted to determine the levels of LAMP1, MAP1LC3, and SQSTM1(P62) and presented in D. Mean ± SEM, n = 3, *p < 0.05. E, F BV2 cells were subjected to subcellular fractionation to determine nuclear translocation TFEB in α-synuclein A53T BV2 cells and presented in F, *p < 0.05. G, H Subcellular localization of TFEB was analyzed by confocal microscopy and presented in H. Scale bars, 10 μm. *p < 0.05. I, J IHC demonstrating decreased TFEB protein levels were rescued by SB203580 in the SNpc of α-synucleinA53T-tg mice. Scale bars, 100 μm. Statistical analysis of the scores of TFEB staining were shown in J. *p < 0.05