Fig. 5

p38 interacts with and phosphorylates TFEB at serine 211. A Lysates from mouse brain were used for IP with anti-TFEB antibody or anti-p38 antibody. B, C Cell lysates from BV2 cells were used for IP with anti-TFEB antibody. SB203580 reduced the TFEB/p38 interaction. *p < 0.05. D, E Cell lysates from BV2 cells were used for IP with anti-p38 antibody. SB203580 reduced the TFEB/p38 interaction and shown in E. Mean ± SEM, n = 3. *p < 0.05. F, G Cell lysates from BV2 cells were used for IP with anti-GFP antibody. SB203580 reduced the TFEB–GFP/14-3-3 interaction. Mean ± SEM, n = 3. *p < 0.05 (H, I). Subcellular localization of TFEB was analyzed by confocal microscopy and shown in I. Scale bars, 10 μm. Mean ± SEM, n = 3, *p < 0.05. J, K BV2 cells were labeled with lysosome tracker and visualized lysosome biogenesis under a microscope and shown in K. Scale bars, 100 μm. Mean ± SEM, n = 10, *p < 0.05. L, M Cell lysates from BV2 cells were immunoblotted to determine the levels of NLRP3, ASC, cleaved CASP1, CathB and shown in M. Mean ± SEM, n = 3, *p < 0.05