Fig. 7
From: A new method for obtaining bankable and expandable adult-like microglia in mice
NEL-MG showed a re-expansion capacity. A GFP-expressing NEL-MG were plated on a neuroepithelial cell feeder and cultured. GFP-expressing NEL-MG were obtained from B6-EGFP mice using our methodology. GFP-expressing Ki67-positive NEL-MG (white line square) or no GFP-expressing Ki67-positive cells (neuroepithelial cells, dot line square) were examined in the immunofluorescence study. B According to the flow cytometry results, the relative ratio of GFP-positive cells was not changed over the culture duration but Ki67-positive GFP-expressing NEL-MG (white line square) were increased over time in immunofluorescence study. Scale bar = 100 μm. **p < 0.01 compared to 3d