Fig. 3

Visceral sensitivity normalizes on day 12 of the DSS-induced colitis. Visceral sensitivity was examined in littermate wild-type mice untreated (Day 0, white), treated 5 days with 3% DSS (Day 5, grey) or treated with 3% DSS for 5 days and then with water until day 12 (Day 12, black) by measuring visceromotor response (VMR) to colorectal distension (n = 9–18 mice/group) (A). B Representative traces of abdominal muscle contractions in response to 60 mm Hg colorectal distension in mice at day 0 (upper panel), day 5 (middle panel) and day 12 (lower panel) of the treatment. The arrows represent the 10 s of distension. Area under the curve (AUC) calculated by plotting individual VMR is shown in C. Data are expressed as mean ± SEM. Statistical analysis was performed using repeated-measures two-way ANOVA and subsequent Sidak’s multiple comparison tests (A) or Kruskal–Wallis and subsequent Dunn’s multiple comparison tests (C). **p < 0.01, ***p < 0.001 compared to normal control mice (D0)