Fig. 5
DOR deletion in Nav1.8-expressing sensory neurons abolishes analgesia on day 12 of the DSS-induced colitis. Visceral sensitivity of conditional knockout mice (black symbols) for either MOR (MOR cKO) (A–C) or DOR (DOR cKO) (D–F) was compared to their corresponding littermate wild-type floxed mice (MOR flox and DOR flox) (white symbols) on day 12 of DSS treatment (n = 5–7 mice/group). A and D Visceromotor response (VMR) to colorectal distension. B and E Representative traces of abdominal muscle contractions in response to 60 mm Hg colorectal distension in littermate floxed mice and conditional KO mice. The arrows represent the 10 s of distension. C and F Area under the curve (AUC) calculated by plotting individual VMR. Data are expressed as mean ± SEM. Statistical analysis was performed using repeated-measures two-way ANOVA and subsequent Sidak’s multiple comparison tests (A and D) or Mann–Whitney U tests (C and F). *p < 0.05, **p < 0.01, ***p < 0.001