Fig. 2
Syk-deficiency does not alter neutrophil accumulation in the acutely injured spinal cord. A Flow cytometry gating of leukocytes (CD45+), myeloid lineage cells (CD11b+), neutrophils (Ly6Clow/Ly6G+), and monocytes (Ly6G−) in the blood and spinal cord at 1 days post-SCI. Microglia were identified in the spinal cord as CD11b+/Ly6G−/CD45low cells. B Neutrophils from the blood and spinal cord of Sykf/fMRP8-Cre show robust Syk-deficiency relative to Sykf/f controls at 1 days post-SCI. Two-way ANOVA with Tukey’s post hoc test. C Syk-deficiency does not alter the proportion of myeloid lineage cells in the peripheral blood compartment at 1 days post-SCI. D No differences were observed in the infiltration of leukocytes or myeloid lineage subtypes at 1 days post-SCI. N = 7 for Sykf/f (4F/3M) and 6 for Sykf/fMRP8-Cre (3F/3M) for B–D. Unpaired two-tailed Student’s t test for C–D. E No differences were observed in the number of neutrophils (Ly6G+ cells) in sections spanning the lesion site in Sykf/f and Sykf/fMRP8-Cre mice. F–H No differences were found in the accumulation of neutrophils in the grey matter, white matter, or meninges of Sykf/f and Sykf/fMRP8-Cre mice. N = 5 for Sykf/f (3F/2M) and 5 for Sykf/fMRP8-Cre (2F/3M) for E–H. Repeated measures two-way ANOVA with Bonferonni’s post hoc test. *p < 0.05, ***p < 0.001. SCI spinal cord injury