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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Spleen tyrosine kinase facilitates neutrophil activation and worsens long-term neurologic deficits after spinal cord injury

Fig. 3

Syk reduces neutrophil activation, but does not mediate neutrophil effector functions, in the acutely injured spinal cord. A Similar CD62L levels (G-MFI) were detected between Sykf/f and Sykf/fMRP8-Cre mice in the blood at 1 days post-SCI. B Syk-deficiency increased CD62L levels on neutrophils, but not other myeloid cell lineages, in the spinal cord of Sykf/fMRP8-Cre mice relative to Sykf/f controls at 1 days post-SCI. N = 7 for Sykf/f (4F/3M) and 6 for Sykf/fMRP8-Cre (3F/3M) for A-B Unpaired two-tailed Student’s t test for A–B. C Similar ROS levels, as measured by DHR123 G-MFI, were observed in blood or spinal cord neutrophils of Sykf/f and Sykf/fMRP8-Cre mice at 1 days post-SCI. N = 5 for Sykf/f (2F/3M) and 4 for Sykf/fMRP8-Cre (1F/3M). D No differences in the proportion of degranulated (MRP8low/−) neutrophils in blood or spinal cord were found between Sykf/f and Sykf/fMRP8-Cre mice at 1 days post-SCI. N = 7 for Sykf/f (3F/4M) and 6 for Sykf/fMRP8-Cre (2F/4M). E Syk-deficiency did not alter the proportion of neutrophils undergoing NETosis (CitH3+) in blood or spinal cord of Sykf/f vs. Sykf/fMRP8-Cre mice at 1 days post-SCI. N = 8 for Sykf/f (5F/3M) and 8 for Sykf/fMRP8-Cre (3F/5M). Two-way ANOVA with Tukey’s post hoc test for C–E. F Immunoblot for extravasated mouse IgG heavy and light chain subunits in the spinal cord at 1 days post-SCI. GAPDH was used as an internal control. No differences were observed in the levels of extravasated mouse IgG heavy or light chain subunits between Sykf/f and Sykf/fMRP8-Cre mice. N = 5/genotype (2F/3M). Unpaired two-tailed Student’s t test for F. *p < 0.05. **p < 0.01, ***p < 0.001. G-MFI geometric mean fluorescent intensity, ROS reactive oxygen species, SCI spinal cord injury, NET neutrophil extracellular trap

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