Fig. 4
Syk augments neutrophil cell death in the acutely injured spinal cord independent of CD11b levels. A Representative flow cytometry histograms of ZombieDye levels in blood and spinal cord neutrophils. Syk deficiency reduced the proportion of intraspinal neutrophils labeled with ZombieDye at 1 days post-SCI. N = 7 for Sykf/f (4F/3M) and 6 for Sykf/fMRP8-Cre (3F/3M). B No differences in CD11b levels (G-MFI) were found between Sykf/f and Sykf/fMRP8-Cre mice at 1 days post-SCI. N = 5 for Sykf/f (2F/3M) and 4 for Sykf/fMRP8-Cre (1F/3M). Two-way ANOVA with Tukey’s post hoc test for A, B. **p < 0.01, ***p < 0.001. G-MFI geometric mean fluorescent intensity, SCI spinal cord injury