Spleen tyrosine kinase facilitates neutrophil activation and worsens long-term neurologic deficits after spinal cord injury

Table 2 Gene expression (ΔCT) in blood and spinal cord neutrophils

Gene symbol	Syk^f/f ΔCT values			Syk^f/fMRP8-Cre ΔCT values		FDR q value
Gene symbol	UN	Blood	Spinal Cord	Blood	Spinal cord	FDR q value
Bcl2a1	9.22	8.86	4.18	7.80	4.53	^bq < 0.001
C3	1.52	1.72	0.01	1.83	− 0.05
Ccl3	10.11	8.43	1.14	8.53	1.82	^bq < 0.001, ^dq = 0.026
Ccl4	7.11	7.61	1.68	7.3	1.75	^bq < 0.001
Ccl9	10.55	9.37	6.84	9.82	7.58	^bq < 0.01
Cox2	8.98	8.36	5.67	7.84	5.49	^bq < 0.01
Csf1	9.93	10.45	5.78	9.58	7.76	^bq < 0.05, ^aq = 0.005
Cxcl2	10.00	8.31	1.82	8.83	2.30	^bq < 0.001, ^dq = 0.025
Cxcl3	11.03	10.18	− 0.23	11.12	0.25	^bq < 0.001
Cxcl10	8.38	9.49	7.27	10.08	6.47	^bq < 0.01
Cxcr4	0.81	0.63	1.19	0.16	0.73
Ddit4	8.45	7.38	4.69	7.27	4.98	^bq < 0.01
Il1a	14.38	14.83	8.14	13.18	8.95	^bq < 0.001, ^cq = 0.013
Il1b	− 1.50	− 1.43	− 0.12	− 1.70	− 0.56
Il1rn	2.71	3.41	− 0.10	2.94	0.07	^bq < 0.001
Il12a	10.78	8.85	9.26	9.34	10.41
Ltb4r1	2.79	2.50	1.43	2.77	1.34
Mdk	12.69	11.70	9.06	11.25	9.28	^bq < 0.01
Mif	4.05	3.59	0.95	3.80	1.07	^bq < 0.001
Osm	9.72	10.40	9.95	10.70	10.43
P2rx7	12.61	12.22	10.43	12.18	10.23	^bq < 0.05
Pcna	6.71	5.99	5.49	5.96	5.61
Slpi	− 0.77	− 0.97	1.19	− 1.50	0.78	^bq < 0.01
Tgfb1	4.26	4.24	3.99	3.74	3.97
Tnfa	8.43	8.44	5.36	8.68	5.76	^bq < 0.001
Tnfaip3	6.89	7.08	4.33	6.93	4.24	^bq < 0.001
Vegfa	11.77	12.48	9.29	11.68	11.04	^bq < 0.01, ^aq = 0.021
Vegfb	8.21	7.99	7.58	7.57	7.42

Values represent average ΔCT for each group. For each sample, CT values were normalized to the average of Gapdh, Actb, and Hprt1. N = 3/group. Two-way ANOVA with Benjamini, Krieger, and Yekutieli multiple comparisons correction
FDR false discovery rate
^aq < 0.05 for spinal cord neutrophils between genotypes
^bq < 0.05 for blood neutrophils vs. spinal cord neutrophils (Syk ^f/f and Syk^f/fMRP8Cre mice)
^cq < 0.05 for blood neutrophils between genotypes
^dq < 0.05 for UN blood neutrophils vs. SCI blood neutrophils (Syk^f/f mice)

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