Fig. 1

Schematic of behavioural tests performed. In summary, animals were exposed to 1% sucrose solution during the 5 days of acclimatisation. On the day of the experiment, male, CD-1 mice received an intraperitoneal injection of R848 (200 μg, dissolved in DMSO and sterile saline) or control solution (DMSO diluted in sterile saline), together with an intravenous injection of nafamostat (3 mg/kg) or vehicle (sterile saline). For the duration of the experiment, animals underwent the sucrose preference test (SPT), and food intake was examined. 4-h post-challenge, animals completed the open field (OF) paradigm. 6 h post-challenge, animals completed the forced swim test (FST), after which their temperature was determined and animals were culled. d = days, hr = hours