Fig. 2
From: Pericytes as mediators of infiltration of macrophages in multiple sclerosis
Confirmation of pericyte heterogeneity using NG2. A Pericytes share markers with other cell type, therefore, to rule out redundancy in the identification of pericytes, we also stained pericytes with NG2 and tracked them along the CD31+ endothelium. Similar to PDGFRβ+ cells, NG2+ pericytes look hypertrophied and elongated across all the time points in EAE cerebellar white matter. Scale 50 µm B Laminin/PDGFRβ and NG2/CD31 staining in chronic EAE (D35) reaffirming the attachment of pericytes to the NVU in later stages of EAE. Scale 50 µm. A 3D rendering using the Imaris image reconstruction software confirms the presence of NG2+ pericytes along the CD31+ vasculature. Scale 6 µm