Fig. 2

Intracranial injection of myelin activates microglia. A Schematic showing the site of intracranial injection and the region of analysis for microglial activation. B–M Tissue sections collected at 24 or 72 h post-injection were immunostained against Iba-1. Representative low magnification images from ipsilateral B, D, F and contralateral C, E, G cortex are shown from animals injected with vehicle (VEH) (B, C), unmodified myelin (UNMOD) (D, E), or citrullinated myelin (CIT) (F, G). Representative higher magnification images at 24 h H–J and 72 h K–M postinjection for mice receiving VEH (H, K), UNMOD myelin (I, L), or CIT myelin (J, M). Scale bar in (G) is 250 μm and refers to (B–G); scale bar in M is 50 μm and refers to (H–M)