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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Citrullinated myelin induces microglial TNFα and inhibits endogenous repair in the cuprizone model of demyelination

Fig. 3

Microglial phagocytosis of myelin is not impacted by citrullination. A pHrodo red-labeled myelin is phagocytosed within 15 min of addition to primary microglia (red = phagocytosed myelin; cells shown in brightfield). BC Phagocytosis continues through 3 h. D By 24 h the total amount of myelin decreased and the cells acquired a larger and more reactive morphology. E Morphological change is maintained through 72 h. F Vehicle-treated cells remain small and largely bipolar. G, H Incubation for 24 h with either unmodified (UNMOD) or citrullinated (CIT) myelin results in the same morphological change relative to vehicle. I Flow cytometric analysis of forward and side scatter profile indicates that both UNMOD and CIT myelin trigger a robust change in cellular size and morphology. J Percent of myelin-laden microglia is the same between UNMOD and CIT conditions. K Uptake and degradation rates are the same for UNMOD and CIT myelin. Graphs show mean ± 95% CI. Scale bar in E is 200 μm and refers to (AE). Scale bar in H is 100 μm and refers to (F–H)

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