Fig. 4

Citrullinated myelin induces a unique transcriptional program in microglia. A Primary microglia were stimulated with vehicle (VEH), unmodified myelin (UNMOD), citrullinated myelin (CIT), or LPS for 24 h and then analyzed by flow cytometry for surface level expression of the activation markers MHCII, TLR2, CD80, and CD86. Red lines provide reference for expression intensity across groups. Graphs show individual experiments (n = 3 for each condition) with mean and standard deviation: V = vehicle; U = unmodified; C = citrullinated; L = LPS. B Heatmap showing z-scores for 691 unique genes clustered by average linkage and Pearson distance. Graphs show reads per kilobase per million mapped reads (RPKM) for a subset of 12 genes with elevated z-scores in microglia treated with CIT myelin relative to both VEH and UNMOD myelin