Fig. 4
Expression of PGD2 synthesis enzymes and an autocrine manner of microglial activation by PGD2. Expression of COX-1 (A), COX-2 (B) L-PGDS (C), and H-PGDS (D) mRNA in the WT hippocampal tissue and primary cultures of Neurons, Astrocytes, and Microglia. Data are mean ± SEM, n = 5 per group. Statistical analysis was performed using one-way ANOVA followed by post-hoc Newman–Keuls test (*p < 0.05; **p < 0.01; ***p < 0.001 vs. Hippocampus). The protein level of Iba-1 in the primary microglia stimulated with PGD2 1 h after addition of DP antagonists (E). Data are mean ± SEM, n = 5 per group. Statistical analysis was performed using one-way ANOVA followed by post-hoc Newman–Keuls test (***p < 0.001 vs. Control, ##p < 0.01; ###p < 0.001 vs. PGD2)