Fig. 11

Identification CD68⁺ and DiI⁺ cells within the lumbar DRGs of WT and Lepr^db/db mice. Sections containing lumbar DRG tissue from WT (A) and Lepr^db/db mice (B) were reviewed to determine the location of CD68⁺DiI⁺ cells. CD68⁺DiI⁺ double positive cells were identified within the DRG, perineural adipose tissue, and meninges surrounding the DRGs. Analysis of the DiI mean signal intensity within the DRG itself revealed a significant difference between WT (n = 3; C) and Lepr^db/db (n = 3; D) mice (p = 0.0167). Images were taken at 20 × and a composite image was formed using the tile scan feature of the Nikon confocal microscope. Scale bar = 100 µm. Error bar represents the standard deviation from three independent measurements