Fig. 12

Identification CD68⁺ and DiI⁺ cells within the spinal column of WT and Lepr^db/db mice. Sections containing lumbar spinal cord tissue from WT (A) and Lepr^db/db mice (B) were reviewed to determine the location of CD68⁺DiI⁺ cells. CD68⁺DiI⁺ double positive cells were identified within the meninges and bone marrow, but not in white matter, grey matter, or nerve roots of the spinal cord. Closer inspection of the meninges revealed CD68⁺DiI⁺ double positive cells within the dura/arachnoid mater (white arrows) and pia mater (green arrows) (C, D). Images were taken at 20 × and a composite image was formed using the tile scan feature of the Nikon confocal microscope. Scale bar = 200 µm (A, B); 100 µm (C, D)