Fig. 8

High magnification images of CD68⁺DiI⁺ double positive cells from hypodermal tissues of the foot in WT and Lepr^db/db mice. Individual CD68⁺DiI⁺ double positive cells were identified in the hypodermis of both WT and Lepr^db/db mice, and high-resolution images were taken using a 100 × objective to confirm colocalization of DiI and CD68 signal within these cells (A). 3D renderings of these confocal images were also generated to demonstrate that the DiI signal was within the CD68⁺ cells (B). Scale bar = 5 µm