Fig. 9

Identification CD68⁺ and DiI⁺ cells within the thighs of WT and Lepr^db/db mice. Histological sections of the left and right thighs from WT (A) and Lepr^db/db (B) mice injected with PFC-NE 72 h prior to tissue collection were subsequently stained with CD68, PGP9.5 and DAPI and reviewed for the distribution of CD68⁺DiI⁺ double positive cells. DiI from the NE was found within CD68⁺ cells in bone marrow (BM), perineural adipose tissue (AT), muscle (M), and sciatic nerve (SN; dotted white line). Images were taken at 20 × and a composite image was formed using the tile scan feature of the Nikon confocal microscope. Scale bar = 250 µm