Fig. 6

Dynamic changes of M1-like and M2-like phenotype microglia in COH retinas of the MacGreen transgenic mice. a Representative fluorescence activating cell sorter (FACS) plots of retinal cells obtained from sham-operated (control, Ctr) and COH MacGreen mice at different post-operational times, assayed by flow cytometry. Microglia in the MacGreen mice express the enhanced green fluorescent proteins. b Representative gating strategy for identifying CD206⁺ and CD86⁺ microglia in retinal cells obtained from Ctr and COH MacGreen mice at different post-operational times. c, d Bar charts showing the changes in the percentage of CD86⁺ M1-like (d) and CD206⁺ M2-like (e) microglia in whole retinal cells obtained from Ctr and those of COH retinas of the MacGreen mice different post-operational times. n = 6 for each group. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. Ctr using ordinary one-way ANOVA with Dunnett’s multiple comparisons test (c, d)