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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis

Fig. 4

Dysregulated tight junctions in the CP affect BCSFB function during early infection. A Tight junctions and B MMP-8, and MMP-13 expression analysis (RT-PCR) of total RNA isolated from CP at 3, 5 and 7 dpi. Data show individual values and mean ± SEM, n = 2–5, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA, with Dunnett’s correction). C Immunofluorescence of coronal brain sections stained for identification of Claudin-2 (green) and ZO-1 (red) tight junctions from naïve and 7 dpi mice. White squares identify the regions of interest shown in higher magnification in the white upper squares. Lower panels indicate the respective inverted separated channels for each staining without DAPI to visualize the protein disturbances. Scale bars = 50 µm. D Functional FITC-dextran permeability assay comparing the leakage of the BCSFB (blue) versus BBB (red) throughout the course of infection. Data show mean ± SEM (n = 5) for each time-point analyzed. ****p < 0.0001, #p < 0.05, ####p < 0.0001. # indicates significant difference between BCSFB and BBB from the same time-point (multiple t-test), and * indicates significant differences between day 0 and the time-point being analyzed from the same barrier type (one-way ANOVA, with Dunnett’s correction)

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