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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: SNHG15 is a negative regulator of inflammation by mediating TRAF2 ubiquitination in stroke-induced immunosuppression

Fig. 2

Validation of SNHG15 expression and localization. The proportions of positive isolated monocytes (A) and negative isolated lymphocytes (B) after sorting by magnetic beads were determined by flow cytometry. Expression of IL-4 (C), IL-10 (D), IL-6 (E), and TNF-α (F) in monocytes/macrophages isolated from PBMCs was detected by qRT-PCR and calculated with ΔCT. G SNHG15 relative expression in subpopulations of PBMCs. The expression of SNHG15 in IL-4-induced (H) or LPS-induced (I) monocytes isolated from PBMCs. J The colocalization of SNHG15 and CD14 + monocytes was assessed by FISH. K SNHG15 expression in the cytoplasm and nucleus of monocytes/macrophages. * P < 0.05, ** P < 0.01, *** P < 0.001

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