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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytes

Fig. 3

Primary human astrocytes downregulate known targets of miR-210 following transfection with miR-210-Mimic. A Primary human astrocytes were transfected with AlexaFluor BLOCK-iT as a positive control of transfection. Representative images of non-transfected (left) and transfected (right) cells immunostained for GFAP and overlayed with brightfield. Scale bar = 100 µm. B Primary human astrocytes were transfected with miR-210-Mimic (210M) or miR-210-Seed-mutant (210S) as a control. The expression of miR-210 in 210M-transfected relative to 210S-transfected cells was assessed by RT-qPCR, n = 6. C MiRabel analysis of miR-210 shows the five most likely gene-targets of miR-210 based on sequence-specific predicted targeting, with lower MiRabel scores signifying a higher likelihood of being targeted by miR-210. D RT-qPCR assessment of microRNA-210 targets Iron Sulfur Cluster Protein (ISCU, n = 9) and E Cytoglobin (CYGB, n = 8) in 210M-transfected relative to 210S-transfected cells. All data is graphed as mean ± SEM. Each dot represents a separate human sample. Paired t tests were performed for tests of significance. *p < 0.05; **p < 0.01; ***p < 0.001

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