Fig. 2

Dysregulated transcriptional profiles of B cell subsets in AQP4-Ab positive NMOSD patients. A Principal component analysis of transcriptional profiles in B cell subsets obtained from HCs and AQP4-Ab positive NMOSD patients. B Expression profiles of differentially-expressed transcription factors in B cell subsets. Relative expression levels (Z-scores) of genes are shown in a heatmap. C (upper row) Expression levels (FKPM: fragments per kilobase of transcript per million) of PRDM1 and BCL6 in each B cell subset (**Q < 0.01, FDR-adjusted p-value after Benjamini–Hochberg correction for multi-testing). (lower row) Relative expression analyzed by qPCR of PRDM1 and BCL6 in each B cell subset. D Expression levels (FKPM) of BACH2 and PAX5 in naïve B cells. C‒D The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum