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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: MORPHIOUS: an unsupervised machine learning workflow to detect the activation of microglia and astrocytes

Fig. 4

Focal and proximal microglia show morphological changes consistent with activation. The morphologies of control microglia from contralateral hippocampal sections (Contra.), ipsilateral FUS-treated hippocampal sections (Ipsi.), and MORPHIOUS classified distal, proximal (Prox.) and focal subregions within ipsilateral FUS-treated sections were compared. Cellular metrics included IBA1 mean intensity (A), IBA1% area coverage (B), mean soma size (C), mean branch length per cell (D), number of branches per cell (E), and the nearest neighbour distance (F). Groups were analyzed via a mixed linear model. Between-group differences were assessed via a Sidak’s post-hoc test. Significance: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data represent means ± SD; N = 4 per group (Contra., Ipsi., Distal, Prox.) and N = 3 per group (Focal). Contra., contralateral; Ipsi., ipsilateral; FUS, focused ultrasound; Prox., proximal

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