Fig. 4From: MORPHIOUS: an unsupervised machine learning workflow to detect the activation of microglia and astrocytesFocal and proximal microglia show morphological changes consistent with activation. The morphologies of control microglia from contralateral hippocampal sections (Contra.), ipsilateral FUS-treated hippocampal sections (Ipsi.), and MORPHIOUS classified distal, proximal (Prox.) and focal subregions within ipsilateral FUS-treated sections were compared. Cellular metrics included IBA1 mean intensity (A), IBA1% area coverage (B), mean soma size (C), mean branch length per cell (D), number of branches per cell (E), and the nearest neighbour distance (F). Groups were analyzed via a mixed linear model. Between-group differences were assessed via a Sidak’s post-hoc test. Significance: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data represent means ± SD; N = 4 per group (Contra., Ipsi., Distal, Prox.) and N = 3 per group (Focal). Contra., contralateral; Ipsi., ipsilateral; FUS, focused ultrasound; Prox., proximalBack to article page