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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: MORPHIOUS: an unsupervised machine learning workflow to detect the activation of microglia and astrocytes

Fig. 7

Proximal astrocytes co-express Nestin, a marker of activation. Within FUS-treated ipsilateral hippocampal sections, MORPHIOUS identified proximally activated (orange line) astrocytes (A) co-labelled with Nestin (B, C). Pearson correlation was used to colocalize GFAP with Nestin (D). Images (A, B) were taken at 20X magnification. Insets (C1–C3) were taken at 63X magnification. Groups were analyzed via a mixed linear model, and between-group differences were assessed via a Sidak’s Post-hoc analysis. Significance: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data represent means ± SD; N = 4 per group. Scale bar: 100 µm (A, B), 25 µm (C1–C3). Contra., contralateral; GFAP, glial fibrillary acidic protein; Prox., proximal

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