Fig. 8From: MORPHIOUS: an unsupervised machine learning workflow to detect the activation of microglia and astrocytesActivated microglia overlap with activated astrocytes. Proximal and focal clusters of IBA1+ microglia (A) overlap spatially with proximal clusters of GFAP+ astrocytes (B). Cluster sizes are reported as the percentage of the total hippocampal area covered by activated microglia (red) or astrocytes (green) (C). In total, 74.5% of astrocytic clusters overlapped with microglia clusters, while 48.7% of microglial clusters overlapped with astrocytes (brown). (D) Within the same section, cluster sizes for activated microglia and activated astrocytes strongly correlated. The correlation coefficient (R2) was analyzed via linear regression analysis (N = 16). Significance: **** P < 0.0001. Scale bar: 100 µm (A, B). GFAP, glial fibrillary acidic proteins; IBA1, ionized calcium-binding adaptor protein 1Back to article page