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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: MORPHIOUS: an unsupervised machine learning workflow to detect the activation of microglia and astrocytes

Fig. 9

MORPHIOUS identified focal and proximal microglia in the hippocampus of TgCRND8 mice. MORPHIOUS identified focal (red line) and proximal microglia (orange line) (A) in association with amyloid-beta plaques (B). Compared with nonTg and distal microglia, proximal and focal microglia exhibited progressively higher IBA1 immunofluorescence (C). Focal microglia showed increased percent area coverage when compared with all other groups (D). Pearson correlation analysis demonstrates that proximal (E) and focal (F) microglial cluster sizes correlated with amyloid plaque load. Activated microglial clusters were associated with greater overall plaque coverage (G) and mean plaque size (H). Images (A, B) were taken at 20X magnification. Between-group differences were assessed via a one-way ANOVA with Tukey’s post-hoc analysis. Correlations were assessed via linear regression analysis and the Pearson correlation coefficient (R2) is reported. Significance: ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data represent means ± SD; N = 4 per group. Scale bar: 100 µm (A, B). IBA1, ionized calcium-binding adapter molecule 1; nonTg, non-transgenic littermates of TgCRND8 mice; Prox., proximal; Tg, TgCRND8 mice

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