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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Sublethal enteroviral infection exacerbates disease progression in an ALS mouse model

Fig. 1

Sublethal CVB3 infection exacerbates ALS-related phenotypes and decreases the lifespan of SOD1G85R mice. A Schematic diagram illustrating intracerebroventricular injections of neonatal SOD1G85R and C57BL/6J mice with either 500 pfu of CVB3 or an equal volume of DMEM (mock infection). Mouse motor functions were monitored weekly starting at week 20 post-infection (PI) until the mice were approaching the humane endpoint or at the experimental endpoint of week 60 PI. Mouse tissues were collected at day 10, week 20 and week 60 PI or at the humane endpoint. B Kaplan–Meier plots comparing survival rates among C57BL/6J Mock (n = 12; male = 4 and female = 8), SOD1G85R Mock (n = 10; male = 3 and female = 7); C57BL/6J CVB3 (n = 9; male = 4 and female = 5), and SOD1G85R CVB3 (n = 8; male = 2 and female = 6) mice. P = 0.002 between SOD1G85R mice inoculated with CVB3 and mock-infected. C Relative body weight measured weekly after infection. Body weight at day 0 was arbitrarily set to 1. D Mouse motor function measurement, including hindlimb reflex score, inverted grid time, footprint time, and stride distance, assessed weekly after infection. Quantifications are presented as mean ± standard error of the mean (SEM). Statistical analysis was carried out by two-way ANOVA, followed by Bonferroni’s multiple comparison test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001

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