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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Sublethal enteroviral infection exacerbates disease progression in an ALS mouse model

Fig. 3

Sublethal CVB3 infection triggers microglia/astrocyte activation and immune infiltration in mice. Representative images of GFAP, Iba1, CD19 and NK1.1 immunohistochemical staining in the hippocampus regions of the brain from mock- or CVB3-infected C57BL/6J or SOD1G85R mice at day 10 PI (A) or week 60 PI (or humane endpoint) (B). Similar observation was made in the regions of infected cerebral cortex (data not shown). Images for other targets (CD68, CD79A, CD4, can CD8) can be found in the Additional file 2: Fig S2. Immune infiltrations were quantified by optical density based on the IHC staining in the hippocampus and cerebral cortex regions. Black boxes on the top right illustrate the enlarged images. Quantifications are presented as mean ± SEM (n = 3–4 individual mouse brain samples for each target, timepoint and experimental condition). Scale bar = 100 μm. Statistical analysis was carried out using two-way ANOVA, followed by Bonferroni’s multiple comparison test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001

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