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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: An overlooked subset of Cx3cr1wt/wt microglia in the Cx3cr1CreER-Eyfp/wt mouse has a repopulation advantage over Cx3cr1CreER-Eyfp/wt microglia following microglial depletion

Fig. 4

Cx3cr1highCre−Eyfp− microglia are Cx3cr1wt/wtCre−Eyfp−. a, b DNA electrophoresis of PCR product from EYFP− and EYFP+ microglia. c Normalized coverage of the first 290 bps in exon 2 of Cx3cr1 in both Cx3cr1highCre−Eyfp− and Cx3cr1CreER-Eyfp/wtCre+Eyfp+ microglia, indicating the integrity of the protein-coding exon 2 of Cx3cr1, mouse 1 and mouse 2 are two biological replicates. d, e Whole-genome sequencing of Cx3cr1highCre−Eyfp− microglia (d) and Cx3cr1CreER-Eyfp/wtCre+Eyfp+ microglia (e). The top panel represents the sequencing mapping on the CreERT2-Eyfp vector; the middle panel represents the sequencing mapping on chromosome 9; the bottom panel is a schematic graph showing the homozygosity of the exon 2 of Cx3cr1

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