Fig. 5

TRPV4 knockdown attenuated MPTP-induced ER stress in the SN. After infused with 0.5 μl AAV-TRPV4 shRNAi bilaterally into the SN of mice for 21 days and administrated with MPTP at 30 mg/kg/day for 7 days, the SN was rapidly dissected out. The protein levels of SERCA2, GRP78, GRP94, CHOP and procaspase-12 in the SN of mice were detected by Western blot. A Knockdown of TRPV4 restored the MPTP-induced decrease in SERCA2 (n = 6). B Knockdown of TRPV4 inhibited the MPTP-induced upregulation of GRP78 (n = 6). C Knockdown of TRPV4 inhibited the MPTP-induced upregulation of GRP94 (n = 6). D Knockdown of TRPV4 inhibited the MPTP-induced upregulation of CHOP (n = 6). E Knockdown of TRPV4 partly inhibited the MPTP-induced decrease in procaspase-12 (n = 6). All data are presented as mean ± SEM. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001