Fig. 1

Workflow of cyclic multiplex fluorescent immunohistochemistry and machine learning-based quantitative image analysis. a Schematic of the cyclic multiplex fluorescent immunohistochemistry protocol, where (1) antibodies were denatured by microwave treatment in boiling citrate buffer for 20 min and (2) fluorophores were quenched by immersion in an oxidizing alkaline solution for 30 min (see details in Methods). b Flowchart of the quantitative image analysis and machine learning pipeline