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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: AMPK-autophagy-mediated inhibition of microRNA-30a-5p alleviates morphine tolerance via SOCS3-dependent neuroinflammation suppression

Fig. 6

Autophagy inhibited the expression of microRNA-30a-5p by degrading DICER and AGO2. BV-2 cells were transfected with 100 pmol Atg5 siRNA or negative control (NC) siRNA for 36 h and then subjected to rapamycin (200 nM) for 12 h. Representative western blot data show the protein levels of LC3-II, DICER, AGO1, AGO2 and SOCS3 (a-e) (n = 3). f The level of miRNA-30a-5p was tested by real-time PCR (n = 3). BV-2 cells were treated with bafilomycin (20 nM) for 12 h, followed by rapamycin administration (200 nM) for 12 h. The protein levels of DICER, AGO1, AGO2 and SOCS3 were evaluated by western blot (gj) (n = 3). k The level of miRNA-30a-5p was tested by real-time PCR (n = 3). Data were analyzed by one-way ANOVA. *p < .05, **p < .01, ***p < .001 vs. the control group; #p < .05, ##p < .01, ###p < .001 vs. the rapamycin-treated group

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