The frequency of cytokine-producing TLR2+ and TLR4+ T cells from CCM patients. In (B), the mean proportion of CD4+ and CD8+ T cells positive for TLR2 and TLR4, as well as (D) MFI of TLR2 and TLR4 for these cells, was determined by cytometry following representative dot-plots and histograms shown in figures A and C after acquisition of 200,000 to 300,000 events in samples obtained from asymptomatic (CCMAsympt, n = 14) and symptomatic (CCMSympt, n = 23) CCM patients. The mean percentage of these cells positive for IL-6 (E), IL-17 (F), IFN-γ (G), and IL-10 (H) was determined by flow cytometry (figure A) in the absence of stimuli (none) or 24 h after activation with Pam3Csk4 (Pam3C, 1 μg/mL) or LPS (100 ηg/mL). Data are shown as mean ± SD of seven independent experiments with 5 to 6 samples per experiment. Significance was calculated by comparing different cell culture conditions from CCMAsympt and CCMSympt groups, and the p values are shown in the figure (B and D, Ordinary ANOVA test and Turkey test; E to H, Kruskal-Wallis test and Dunn’s test).