Skip to main content
Fig.3 | Journal of Neuroinflammation

Fig.3

From: Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway

Fig.3

5-HT2BR is required for the effects of fluoxetine on activation of A1 astrocyte in vitro. A Expression of 5-HT2BR on primary cultured microglia, astrocyte and neuron. B Expression of 5-HT2BR on astrocyte in the hippocampus. Similar results were obtained in 3 separate experiments. C, D Effects of 5-HT2BR inhibitor, RS127445, on the expression of A1-special transcripts in astrocytes with or without fluoxetine treatment after A1 cocktail C or IL-6 D stimulation for 24 h. E Effects of 5-HT2BR knockdown on the mRNA levels of A1-special markers in astrocytes with or without fluoxetine treatment after IL-6 stimulation for 24 h. Scale bars, 20 µm in B. Quantitative data are mean ± s.e. Data were analyzed using two-way ANOVA, then combined with Tukey’s multiple comparison to assess the differences between groups. All data represent the results of three independent experiments. CE: **P < 0.01, and ***P < 0.001 vs respective Control cells treated with PBS; #P < 0.05, ##P < 0.01, and ###P < 0.001 vs Control cells treated with A1 (C) or IL-6 (D, E) stimulation; $P < 0.05, $$P < 0.01, and $$$P < 0.001

Back to article page